Amidation (in vivo) is a common post-translational modification in peptides and peptide hormones in which the N-Cα bond of a C-terminal glycine is cleaved in two steps by the bifunctional enzyme PAM, resulting in an amidated C-terminus. Lit.



Type
Predefined
pKa1
None
pKa2
None
pKa3
None
NativeCharge
Loss
C2H3O2
Gain
H
Deltamass
Monoisotopic
-58.0054793032652
Average
-58.0361631558747
UVSpec
λ
/ nm 		ε
/ M-1 cm-1
214 0
240 0
245 0
250 0
255 0
260 0
265 0
270 0
272 0
274 0
276 0
278 0
280 0
282 0
284 0
286 0
288 0
290 0
292 0
294 0
296 0
298 0
300 0
302 0
304 0
306 0
308 0
310 0
312 0
314 0
316 0
318 0
320 0
H
-3
AA
$
Pattern
(?<=G)$