Amidation (in vitro) is an artificial modification that can be introduced at the C-terminus of a protein whereby the carboxyl group is replaced by an amide group. The C-terminal amide can be formed by chemical synthesis or by catalysation of carboxypeptidase-Y.

Type

Predefined

pKa1

None

pKa2

None

pKa3

None

NativeCharge

Loss

Gain

NH₂

Deltamass

Monoisotopic
-0.984015582630597
Average
-0.984760961507241

UVSpec

Display

λ / nm	ε / M^-1 cm^-1
214	0
240	0
245	0
250	0
255	0
260	0
265	0
270	0
272	0
274	0
276	0
278	0
280	0
282	0
284	0
286	0
288	0
290	0
292	0
294	0
296	0
298	0
300	0
302	0
304	0
306	0
308	0
310	0
312	0
314	0
316	0
318	0
320	0

H

-3

AA

Pattern

λ / nm	ε / M^-1 cm^-1
214	0
240	0
245	0
250	0
255	0
260	0
265	0
270	0
272	0
274	0
276	0
278	0
280	0
282	0
284	0
286	0
288	0
290	0
292	0
294	0
296	0
298	0
300	0
302	0
304	0
306	0
308	0
310	0
312	0
314	0
316	0
318	0
320	0

λ / nm	ε / M^-1 cm^-1
214	0
240	0
245	0
250	0
255	0
260	0
265	0
270	0
272	0
274	0
276	0
278	0
280	0
282	0
284	0
286	0
288	0
290	0
292	0
294	0
296	0
298	0
300	0
302	0
304	0
306	0
308	0
310	0
312	0
314	0
316	0
318	0
320	0

λ / nm	ε / M^-1 cm^-1
214	0
240	0
245	0
250	0
255	0
260	0
265	0
270	0
272	0
274	0
276	0
278	0
280	0
282	0
284	0
286	0
288	0
290	0
292	0
294	0
296	0
298	0
300	0
302	0
304	0
306	0
308	0
310	0
312	0
314	0
316	0
318	0
320	0