Alkylation (iodoacetic acid)

Iodoacetic acid reacts covalently with the side-chain thiol group of cysteine. It is often used to modify SH-groups to prevent the re-formation of disulfide bonds after the reduction of cystine residues to cysteine.



Type
Predefined
pKa1
Acidic
3.2
pKa2
None
pKa3
None
NativeCharge
Loss
H
Gain
C2H3O2
Deltamass
Monoisotopic
58.0054793032652
Average
58.0361631558747
UVSpec
Display
λ
/ nm
ε
/ M-1 cm-1
214 0
240 0
245 0
250 0
255 0
260 0
265 0
270 0
272 0
274 0
276 0
278 0
280 0
282 0
284 0
286 0
288 0
290 0
292 0
294 0
296 0
298 0
300 0
302 0
304 0
306 0
308 0
310 0
312 0
314 0
316 0
318 0
320 0
H
-1.7
AA
C
Pattern