Nonenzymatic deamidation of asparagines is a common modification of proteins. Deamidation proceeding through the formation fo succinimide intermediate followed by hydrolysis results in the formation of isoaspartate and aspartate in a molar ratio of approximately 3:1.

Type

Predefined

pKa1

Acidic
4.07

pKa2

None

pKa3

None

NativeCharge

Loss

NH₃

Gain

H₂O

Deltamass

Monoisotopic
0.984015582630597
Average
0.984760961507245

UVSpec

Display

λ / nm	ε / M^-1 cm^-1
214	0
240	0
245	0
250	0
255	0
260	0
265	0
270	0
272	0
274	0
276	0
278	0
280	0
282	0
284	0
286	0
288	0
290	0
292	0
294	0
296	0
298	0
300	0
302	0
304	0
306	0
308	0
310	0
312	0
314	0
316	0
318	0
320	0

H

4.08

AA

Pattern

λ / nm	ε / M^-1 cm^-1
214	0
240	0
245	0
250	0
255	0
260	0
265	0
270	0
272	0
274	0
276	0
278	0
280	0
282	0
284	0
286	0
288	0
290	0
292	0
294	0
296	0
298	0
300	0
302	0
304	0
306	0
308	0
310	0
312	0
314	0
316	0
318	0
320	0

λ / nm	ε / M^-1 cm^-1
214	0
240	0
245	0
250	0
255	0
260	0
265	0
270	0
272	0
274	0
276	0
278	0
280	0
282	0
284	0
286	0
288	0
290	0
292	0
294	0
296	0
298	0
300	0
302	0
304	0
306	0
308	0
310	0
312	0
314	0
316	0
318	0
320	0

λ / nm	ε / M^-1 cm^-1
214	0
240	0
245	0
250	0
255	0
260	0
265	0
270	0
272	0
274	0
276	0
278	0
280	0
282	0
284	0
286	0
288	0
290	0
292	0
294	0
296	0
298	0
300	0
302	0
304	0
306	0
308	0
310	0
312	0
314	0
316	0
318	0
320	0