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Use a subunit for each chain. This is important for the correct calculation of the mass and pI.
On Isoelectric point calculation of native proteins
The charge z (actually the charge number) has no unit. Multiply the charge (number) z by the elementary charge (e)…
On Isoelectric point calculation of native proteins
If you are not sure which algorithm to use, you are certainly well advised to use ProMoST. It usually provides…
On Isoelectric point calculation of native proteins
Dear Reguinaldo For denatured proteins it is best to use the "ProMoST" option, which is the standard.
On Isoelectric point calculation of native proteins
Release of version 2.2.28 Updates: Fixed: Identical id in Peptide ToolKnown issue: Downloaded bitmap graphics shows rendering issue
On Mass spectrum simulator in Prot pi version 2.2
Thanks for reporting this! The issue was caused by an outdated setting, which is now fixed.
On Isoelectric point calculation of native proteins
Release of versoin 2.2 27 Fix: Download graphics results in 400 bad request fixed Known issue: Downloaded bitmap graphics shows…
On Mass spectrum simulator in Prot pi version 2.2
Release of version 2.2.26 Updates: Updated librariesShortened bonds in glycosylation
On Mass spectrum simulator in Prot pi version 2.2
Release of version 2.2.25 Bug Fix: pKa 2 and 3 of custom modification did not work correctely. This works now.…
On Mass spectrum simulator in Prot pi version 2.2
Release of Version 2.2.24 Updates: Optimised modification database Updated frameworks
On Mass spectrum simulator in Prot pi version 2.2