Mass spectrum simulator in Prot pi version 2.2

A new mass spectrum simulator comes with the current version 2.2 of Prot pi. This simulator brings some weighty benefits such as to define the ion adduct, charge state and resolution. An awesome feature is the possibility to integrate multiple compounds in one mass spectrum. This gives amongst others the opportunity to simulate multiple charge state of a compound. In […]

A mass spectrometry imaging software: Quantinetix™

ImaBiotech, founded in 2009 by a team of experts, offers new molecular imaging (called MALDI imaging) services and products to life science industries. This new technology is capable of visualizing and quantifying biological compounds that play role in many pathologies like Cancer, Alzheimer or Parkinson diseases. It is also an interesting tool to follow new […]

Fast ultra-high resolution mass spectrum simulation with Prot pi version 2.1

Version 2.1 of the Protein Tool comes with a completely new software architecture. Even ultra-high resolution (up to 1’000’000) mass spectra of large proteins can now be simulated within sub-seconds. To get the mass spectrum, Prot pi calculates the accurate isotopic distribution using isotope-frequencies and mass defects of the chemical elements. Also the overall calculation time […]

MS-based Glycan Analysis of the Glycoprotein Ricin

1  Background N-glycosylations affect important properties such as the specificity, efficacy, immunogenicity and stability of proteins. A portfolio of methods for glycan analysis was developed, using the murine monoclonal antibody 1RK1, an IgG1κ, to enable N-glycosylations of proteins to be analysed (Fig. 1). The methods developed were subsequently applied to the plant glycoprotein ricin.

MALDI-TOF-MS analysis of antibody-antigen complexes

Editor: Prof. Dr. Christiane Zaborosch Head of Center for Biochemistry Lecturer in Biochemistry  Introduction In the development of new drugs, it is of interest in pharmaceutical research to determine the size of antibody-antigen complexes that have formed, since it is assumed that large complexes are cleared more quickly by the body, and thus the […]