Molecular mass and isoelectric point calculator for glycosylated proteins

Glycosylations should not be neglected for the correct calculation of the molecular mass, the isoelectric point and the mass-specific UV absorption coefficient. Therefore Prot pi provides a tool to draw glycans as a posttranslational modification of proteins. This short guide deals with how to add two complex-type N-Glycosylation G1 with a sialic acid (N-acetylneuraminic acid) […]

Isoelectric point calculation of native proteins

The isoelectric point (pI) of native (not denaturated) proteins is important in several separation techniques. The pI is defined as the pH value at which the positive and negative charges on the protein are balanced and the net charge is zero. To determine the pH at which the net charge is zero, the charge on […]

Encrypted data transfer enabled on Prot pi

To protect your valuable data, Prot pi is now reachable via HTTPS with secure sockets layer (SSL) encryption. Therefore the domain was migrated to a server with a static IP address and a security certificate was issued. The reason for this is to keep your sensitive information sent across the internet encrypted so that […]

MALDI-TOF-MS analysis of antibody-antigen complexes

Editor: Prof. Dr. Christiane Zaborosch Head of Center for Biochemistry Lecturer in Biochemistry  Introduction In the development of new drugs, it is of interest in pharmaceutical research to determine the size of antibody-antigen complexes that have formed, since it is assumed that large complexes are cleared more quickly by the body, and thus the […]

Bioinformatics (from Wikipedia)

This is the definition from Wikipedia: Bioinformatics is an interdisciplinary field that develops methods and software tools for understanding biological data. As an interdisciplinary field of science, bioinformatics combines computer science, statistics, mathematics, and engineering to study and process biological data. [ratings]

Bioanalysis (from Wikipedia)

  This ist the definition from Wikipedia: Bioanalysis is a sub-discipline of analytical chemistry covering the quantitative measurement of xenobiotics (drugs and their metabolites, and biological molecules in unnatural locations or concentrations) and biotics (macromolecules, proteins, DNA, large molecule drugs, metabolites) in biological systems. [ratings]

Prot pi (Alpha) ist online

Vielen Dank für Ihren Besuch auf! Die erste Alphaversion der Bioinformatik-Plattform Prot pi ging am 11. Oktober 2014 Online. Prot pi ist ein Tool zur Berechnung physiko-chemischer Parameter von Proteinen anhand derer Aminosäure-Sequenz. Mit der aktuellen Alphaversion wird hauptsächlich die Stabilität der Programmierung überprüft. Das Release einer Betaversion mit erweiterter Funktionalität ist anfangs 2015 […]